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Nanogel‐based delivery system enhances PGE 2 effects on bone formation
Author(s) -
Kato Norihiko,
Hasegawa Urara,
Morimoto Nobuyuki,
Saita Yoshitomo,
Nakashima Kazuhisa,
Ezura Yoichi,
Kurosawa Hisashi,
Akiyoshi Kazunari,
Noda Masaki
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21160
Subject(s) - nanogel , chemistry , prostaglandin e , calvaria , bone formation , prostaglandin e2 , drug delivery , saline , bone remodeling , endocrinology , biochemistry , in vitro , medicine , organic chemistry
Abstract Recovery of bone loss is one of the active research issues in bone medicine due to the need for efficient measures for bone gain. We examined here a novel drug delivery system using a nanogel of cholesterol‐bearing pullulan (CHP) in combination with prostaglandin E 2 (PGE 2 ). PGE 2 or PGE 2 /CHP, vehicle (saline containing 0.06% ethanol and 0.02% Tween 80) or CHP were injected on to the calvariae of mice once every day for 5 days per week for 4 weeks. Low dosage of PGE 2 (0.6 µg) alone or CHP alone did not induce new bone formation in this system. In contrast, PGE 2 (0.6 µg)/CHP induced new bone formation. Bone formation activities of PGE 2 was enhanced by CHP nanogels only at the site of injection (calvaria) but not in the distant sites of the skeleton, showing that PGE 2 /CHP could avoid systemic effects. In spite of the fact that previously reported animal models of bone formation by PGE 2 were associated with loss of body weight, bone formation based on PGE 2 /CHP did not associate with loss of body weight. Furthermore, only a single application of PGE 2 in combination with nanogel cross‐linking hydrogel sphere (PGE 2 /CHP‐PEO) induced new bone formation. Thus, nanogel‐based delivery system is an efficient delivery system of bone anabolic agent, PGE 2 . J. Cell. Biochem. 101:1063–1070, 2007. © 2007 Wiley‐Liss, Inc.