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Phenotypic expression of human hepatoma cells in culture
Author(s) -
Cutroneo Kenneth R.,
White Sheryl L.,
Buttolph Thomas R.,
Allison Gretchen,
Ehrlich H. Paul
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21150
Subject(s) - phenotype , hypoxia (environmental) , erythropoietin , hypoxia inducible factors , biology , in vitro , gene , secretion , intracellular , cell culture , gene expression , compartmentalization (fire protection) , microbiology and biotechnology , chemistry , biochemistry , genetics , enzyme , organic chemistry , oxygen
Hepatomas thrive in a hypoxic environment resulting in the induction of a cluster of hypoxia related genes. The protein phenotypic expression include hypoxia inducible factor‐α, prolyl‐4‐hydroxylase, vascular endothelear growth factor and erythropoietin. The present study was undertaken to determine if human hepatoma cells when cultured for 72 h in the presence of serum under normoxia would maintain their cancerous phenotypic expression of certain hypoxia inducible genes. Our positive results affords an in vitro model system to test hypoxia inhibitors on the expression and the intracellular compartmentalization or the secretion of these hypoxia‐inducible proteins. J. Cell. Biochem. 100: 1081–1085, 2007. © 2007 Wiley‐Liss, Inc.