Premium
Suppression of growth factor expression and human vascular smooth muscle cell growth by small interfering RNA targeting EGR‐1
Author(s) -
Fahmy Roger G.,
Khachigian Levon M.
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21145
Subject(s) - small interfering rna , microbiology and biotechnology , cell growth , vascular smooth muscle , growth factor , fibroblast growth factor , biology , rna , cell , cancer research , chemistry , gene , endocrinology , smooth muscle , biochemistry , receptor
Smooth muscle cell (SMC) proliferation and migration are key processes that occur in the reparative response to injury after percutaneous coronary intervention and in failed bypass grafts for the treatment of atherosclerosis. In the present study, we generated novel synthetic small interfering RNA (siRNA) molecules targeting the coding region of human early growth response‐1 (EGR‐1) mRNA that attenuate the expression of EGR‐1 and that of fibroblast growth factor‐2 (FGF‐2) and granulocyte‐colony stimulating factor (G‐CSF). These agents suppressed SMC proliferation in a dose‐dependent and non‐toxic manner and blocked SMC regrowth from the wound edge following mechanical injury in vitro. In contrast, the scrambled counterpart did not inhibit SMC proliferation, EGR‐1 protein expression or SMC regrowth after injury. These findings demonstrate that EGR‐1 siRNA can serve as inhibitors of SMC proliferation and wound repair suggesting that these agents may potentially be useful in the control of vascular proliferative disorders. J. Cell. Biochem. 100: 1526–1535, 2007. © 2006 Wiley‐Liss, Inc.