Premium
Suppressing phosphatidylcholine‐specific phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells
Author(s) -
Wang Nan,
Xie Kun,
Huo Siwei,
Zhao Jing,
Zhang Shangli,
Miao Junying
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21139
Subject(s) - nadph oxidase , mesenchymal stem cell , reactive oxygen species , microbiology and biotechnology , chemistry , intracellular , superoxide dismutase , cellular differentiation , phospholipase c , phospholipase , biology , biochemistry , oxidative stress , signal transduction , enzyme , gene
In the previous research, we found that D609 (tricyclodecan‐9‐yl‐xanthogenate) could induce human marrow stromal cell (hMSC) differentiation to neuron‐like cells. In this study, to understand the possible mechanism, we sequentially investigated the changes of phosphatidylcholine‐specific phospholipase C (PC‐PLC) activity, the expression of Rb, the intracellular reactive oxygen species (ROS) levels, NADPH oxidase and superoxide dismutase (SOD) activities when D609 induced neuronal differentiation in rat mesenchymal stem cells (MSCs). The results showed that D609 obviously inhibited the activity of PC‐PLC when it induced neuronal differentiation in rat MSCs. Simultaneously, ROS level and the activity of NADPH oxidase increased significantly, but the MnSOD and Cu/ZnSOD activities were not altered. Furthermore, the level of Rb protein was evidently elevated. Our data suggested that PC‐PLC mediated neuronal differentiation of rat MSCs by elevating NADPH oxidase activity, ROS level, and up‐regulating the expression of Rb protein. J. Cell. Biochem. 100: 1548–1557, 2007. © 2007 Wiley‐Liss, Inc.