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Structural and biochemical advances in mammalian RNAi
Author(s) -
Collins Robert E.,
Cheng Xiaodong
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21069
Subject(s) - rna interference , gene silencing , biology , rna induced silencing complex , dna methylation , heterochromatin , argonaute , microrna , trans acting sirna , genetics , computational biology , gene , gene expression , microbiology and biotechnology , rna , chromatin
RNAi is a collection of processes mediated by small RNAs that silence gene expression in a sequence‐specific manner. Studies of processes as divergent as post‐transcriptional gene silencing, transcriptional silencing through RNA‐directed DNA methylation, or heterochromatin formation, and even RNA‐guided DNA elimination have converged on a core pathway. This review will highlight recent structural and mechanistic studies illustrating siRNA and miRNA processing, RISC formation, the execution of RNAi by RISC, and the regulation of these pathways, with a specific focus on vertebrate systems. J. Cell. Biochem. 99: 1251–1266, 2006. © 2006 Wiley‐Liss, Inc.