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Involvement of caspase activation and mitochondrial stress in trichostatin A‐induced apoptosis of Burkitt's lymphoma cell line, Akata
Author(s) -
Son YoungOk,
Choi KiChoon,
Lee JeongChae,
Kook SungHo,
Lee HyunJeong,
Jeon YoungMi,
Kim JongGhee,
Kim Ju,
Lee WonKeun,
Jang YongSuk
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21022
Subject(s) - apoptosis , trichostatin a , microbiology and biotechnology , cell culture , epstein–barr virus , chemistry , burkitt's lymphoma , caspase , cell growth , histone deacetylase inhibitor , biology , programmed cell death , cancer research , histone deacetylase , immunology , virus , histone , biochemistry , dna , genetics
Epstein–Barr virus (EBV) infects more than 90% of the human population and has a potential oncogenic nature. Trichostatin A (TSA) has potent antitumor activity, but its exact mechanism on EBV‐infected cells is unclear. This study examined the effects of TSA on proliferation and apoptosis of the Burkitt's lymphoma cell line, Akata. TSA treatment inhibited cell growth and induced cytotoxicity in both the EBV‐negative and ‐positive Akata cells. TSA sensitively induced apoptosis in both cells, as demonstrated by the increased number of positively stained cells in the TUNEL assay, the migration of many cells to sub‐G 1 phase by flow cytometric analysis, and the formation of DNA ladders. This suggests that EBV has no effect on the sensitivity to TSA. Western blot analysis showed that the cleavage of PARP and Bid and the activation of caspases are closely related to the TSA‐induced apoptosis of the cells. The reduction in mitochondrial transition potential and the release of apoptosis‐inducing factor from mitochondria to cytosol was also observed after the TSA treatment, but was suppressed by treating the cells with a cathepsin B inhibitor. Overall, these findings suggest that besides the caspase‐dependent pathway, mitochondrial events are also associated with the TSA‐induced apoptosis of Akata cells. J. Cell. Biochem. 99: 1420–1430, 2006. © 2006 Wiley‐Liss, Inc.