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Plasma membrane destination of the classical Xenopus laevis progesterone receptor accelerates progesterone‐induced oocyte maturation
Author(s) -
Martinez Silvana,
Grandy Rodrigo,
Pasten Pamela,
Montecinos Hernán,
Montecino Martín,
Olate Juan,
Hinrichs María Victoria
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20941
Subject(s) - oocyte , xenopus , receptor , endocrinology , medicine , progesterone receptor , microbiology and biotechnology , biology , chemistry , genetics , embryo , gene , cancer , estrogen receptor , breast cancer
Xenopus laevis oocyte maturation is induced by the steroid hormone progesterone through a non‐genomic mechanism initiated at the cell membrane. Recently, two Xenopus oocyte progesterone receptors have been cloned; one is the classical progesterone receptor (xPR‐1) involved in genomic actions and the other a putative seven‐transmembrane‐G‐protein‐ couple receptor. Both receptors are postulated to be mediating the steroid‐induced maturation process in the frog oocyte. In this study, we tested the hypothesis that the classical progesterone receptor, associated to the oocyte plasma membrane, is participating in the reinitiation of the cell cycle. Addition of a myristoilation and palmytoilation signal at the amino terminus of xPR‐1 (mp xPR‐1), increased the amount of receptor associated to the oocyte plasma membrane and most importantly, significantly potentiated progesterone‐induced oocyte maturation sensitivity. These findings suggest that the classical xPR‐1, located at the plasma membrane, is mediating through a non‐genomic mechanism, the reinitiation of the meiotic cell cycle in the X . laevis oocyte. J. Cell. Biochem. 99: 853–859, 2006. © 2006 Wiley‐Liss, Inc.