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Differential expression of HNF4α isoforms in liver stem cells and hepatocytes
Author(s) -
Li Keguo,
Zhang Haiquan,
Wang Yanjun,
Wang Yun,
Feng Meifu
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20939
Subject(s) - stem cell , flow cytometry , gene isoform , hepatocyte , microbiology and biotechnology , differential centrifugation , hepatocyte nuclear factor 4 , biology , liver cytology , western blot , blot , chemistry , transcription factor , biochemistry , in vitro , gene , nuclear receptor , liver metabolism
Abstract The hepatocyte nuclear factor (HNF4α) has been implicated in liver development and hepatocellular differentiation. The HNF4α expression in liver stem cells has not yet been fully investigated. Here, we describe the expression characterization of HNF4α in liver stem cells isolated from 2‐acetylaminofluorene/partial hepatectomy (AAF/PH) rat model by a combination of density‐gradient centrifugation and selective enzymatic digestion. The obtained cells were identified by flow cytometry (FCM) immediately and then by immunocytochemisty and reverse transcription‐polymerase chain reaction (RT‐PCR) after 5 d culture. The cultured stem cells were subjected to analysis of the expression of various HNF4α isoforms using RT‐PCR, Western blotting, and electrophoretic mobility shift assay (EMSA). Results showed that HNF4α isoforms, α1, α2, α7, and α8 were present in liver stem cells, which contrasted with only α1 and α2 expression in hepatocytes. The distinct expression patterns of HNF4α isoforms in liver stem cells may contribute to maintain the “stemness” of these cells. J. Cell. Biochem. 99: 558–564, 2006. © 2006 Wiley‐Liss, Inc.