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Endodermal differentiation of murine embryonic carcinoma cells by retinoic acid requires JLP, a JNK‐scaffolding protein
Author(s) -
Kashef Kimia,
Xu Hua,
Reddy E. Premkumar,
Dhanasekaran Danny N.
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20930
Subject(s) - retinoic acid , microbiology and biotechnology , heterotrimeric g protein , p19 cell , embryonic stem cell , cellular differentiation , chemistry , scaffold protein , biology , signal transduction , g protein , induced pluripotent stem cell , biochemistry , gene
Retinoic acid (RA) is a morphogen that induces endodermal differentiation of murine P19 embryonic carcinoma cells. RA‐induced differentiation of P19 cells has been used as a model system to define the differentiation programs of pluripotent stem cells. Using this system it has been shown that Gα 13 —the α‐subunit of the heterotrimeric G protein G 13 —and its activation of JNK‐module are critically required for the endodermal differentiation of P19 cells. However, the mechanism through which Gα 13 is linked to JNK‐module is unknown. Here, we report that RA stimulates the expression of JNK‐interacting leucine zipper protein (JLP), a newly identified JNK‐scaffolding protein and its critical role in RA‐mediated endodermal differentiation. Our results indicate that there is a physical association between JLP and Gα 13 in RA‐stimulated P19 cells. More interestingly, silencing JLP abrogates RA‐mediated endodermal differentiation of P19 cells analogous to the effects seen with the silencing of Gα 13 or JNK. Therefore, our studies presented here identify for the first time, a novel role for a newly identified scaffolding protein in RA‐mediated endodermal differentiation, providing a new signaling conduit to transmit signals from RA to JNK module. J. Cell. Biochem. 98: 715–722, 2006. © 2006 Wiley‐Liss, Inc.