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Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue
Author(s) -
Izadpanah Reza,
Trygg Cynthia,
Patel Bindiya,
Kriedt Christopher,
Dufour Jason,
Gimble Jeffery M.,
Bunnell Bruce A.
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20904
Subject(s) - adipose tissue , mesenchymal stem cell , stem cell transplantation for articular cartilage repair , bone marrow , stem cell , clinical uses of mesenchymal stem cells , microbiology and biotechnology , biology , pathology , chemistry , adult stem cell , medicine , endocrinology , endothelial stem cell , in vitro , biochemistry
The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non‐human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isolated from rhesus bone marrow (rBMSCs) and human adipose tissue (hASCs) had more similar biologic properties than MSCs of rhesus adipose tissue (rASCs) and human bone marrow MSCs (hBMSCs). Analyses of in vitro growth kinetics revealed shorter doubling time for rBMSCs and hASCs. rBMSCs and hASCs underwent significantly more population doublings than the other MSCs. MSCs from all sources showed a marked decrease in telomerase activity over extended culture; however, they maintained their mean telomere length. All of the MSCs expressed embryonic stem cell markers, Oct‐4, Rex‐1, and Sox‐2 for at least 10 passages. Early populations of MSCs types showed similar multilineage differentiation capability. However, only the rBMSCs and hASCs retain greater differentiation efficiency at higher passages. Overall in vitro characterization of MSCs from these two species and tissue sources revealed a high level of common biologic properties. However, the results demonstrate clear biologic distinctions, as well. J. Cell. Biochem. 99: 1285–1297, 2006. © 2006 Wiley‐Liss, Inc.