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Splice variant 3, but not 2 of receptor protein‐tyrosine phosphatase σ can mediate stimulation of insulin‐secretion by α‐latrotoxin
Author(s) -
Lajus Sophie,
Lang Jochen
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20871
Subject(s) - protein tyrosine phosphatase , secretion , receptor , insulinoma , biology , chinese hamster ovary cell , tyrosine , splice , microbiology and biotechnology , stimulation , alternative splicing , biochemistry , endocrinology , insulin , messenger rna , gene
α‐latrotoxin (α‐LTX) binds to several cell surface receptors including receptor protein‐tyrosine phosphatase σ (RPTPσ). Here we demonstrate that transient overexpression of the short splice variant 3 conferred α‐LTX induced secretion to hamster insulinoma (HIT‐15) cells. In contrast, the long splice variant 2 containing four additional extracellular fibronectin‐III domains was inactive in secretion or in a single cell assay. Toxin‐sensitive (MIN6) and toxin‐insensitive (HIT‐T15) insulinoma cell lines as well as PC12 cells expressed similar amounts of endogenous short RPTPσ splice variant suggesting that this receptor does not play a role for toxin‐sensitivity. J. Cell. Biochem. © 2006 Wiley‐Liss, Inc.