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DNA sequences acting as binding sites for NM23/NDPK proteins in melanoma M14 cells
Author(s) -
Cervoni Laura,
Egistelli Lorenza,
Eufemi Margherita,
d'Abusco Anna Scotto,
Altieri Fabio,
Lascu Ioan,
Turano Carlo,
Giartosio Anna
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20808
Subject(s) - chromatin immunoprecipitation , immunoprecipitation , nucleoside diphosphate kinase , gene , microbiology and biotechnology , dna , biology , promoter , transcription factor , chromatin , chemistry , biochemistry , gene expression
We isolated and analyzed by chromatin immunoprecipitation (ChIP) in viable M14 cells DNA sequences bound to the antimetastatic protein nucleoside diphosphate kinase (NM23/NDPK) to shed some light on the nuclear functions of this protein and on the mechanism by which it acts in development and cancer. We assessed the presence of selected sequences from promoters of platelet‐derived growth factor A ( PDGF‐A ), c‐ myc , myeloperoxidase ( MPO ), CD11b , p53 , WT1 , CCR5 , ING1 , and NM23‐H1 genes in the cross‐linked complexes. Quantitative PCR (Q‐PCR) showed a substantial enrichment of the correlated oncosuppressor genes p53 , WT1 , ING1 , and NM23‐H1 in the immunoprecipitated (IP) DNA. This suggests that NM23/NDPK binding is involved in the transcription regulation of these genes. These results reveal new interactions that should help us to disclose the antimetastatic mechanism of NM23. J. Cell. Biochem. 98: 421–428, 2006. © 2006 Wiley‐Liss, Inc.