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In vivo photothermal flow cytometry: Imaging and detection of individual cells in blood and lymph flow
Author(s) -
Zharov Vladimir P.,
Galanzha Ekaterina I.,
Tuchin Valery V.
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20766
Subject(s) - flow cytometry , in vivo , photothermal therapy , pathology , microvessel , blood flow , in vitro , cytometry , lymph node , cell , preclinical imaging , biomedical engineering , microbiology and biotechnology , biology , chemistry , cancer research , immunology , medicine , angiogenesis , nanotechnology , materials science , biochemistry , radiology
Flow cytometry is a well‐established, powerful technique for studying cells in artificial flow in vitro. This review covers a new potential application of this technique for studying normal and abnormal cells in their native condition in blood or lymph flow in vivo. Specifically, the capabilities of the label‐free photothermal (PT) technique for detecting and imaging cells in the microvessel network of rat mesentery are analyzed from the point of view of overcoming the problems of flow cytometry in vivo. These problems include, among others, the influences of light scattering and absorption in vessel walls and surrounding tissues, instability of cell velocity, and cells numbers and positions in a vessel's cross‐section. The potential applications of this new approach in cell biochemistry and medicine are discussed, including molecular imaging; studying the metabolism and pathogenesis of many diseases at a cellular level; and monitoring and quantifying metastatic and apoptotic cells, and/or their responses to therapeutic interventions (e.g., drug or radiation), in natural biological environments. J. Cell. Biochem. 97: 916–932, 2006. © 2006 Wiley‐Liss, Inc.