Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes | Zendy

Au KaWing | Zendy; Kou Cecy Y.C. | Zendy; Woo Anthony Y.H. | Zendy; Chim Stephen S.C. | Zendy; Fung KwokPui | Zendy; Cheng Christopher H.K. | Zendy; Waye Mary M.Y. | Zendy; Tsui Stephen K.W. | Zendy

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Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes

Author(s) -

Au KaWing,

Kou Cecy Y.C.,

Woo Anthony Y.H.,

Chim Stephen S.C.,

Fung KwokPui,

Cheng Christopher H.K.,

Waye Mary M.Y.,

Tsui Stephen K.W.

Publication year - 2006

Publication title -

journal of cellular biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.028

H-Index - 165

eISSN - 1097-4644

pISSN - 0730-2312

DOI - 10.1002/jcb.20710

Subject(s) - myogenesis , downregulation and upregulation , myocyte , microbiology and biotechnology , suppression subtractive hybridization , cellular differentiation , hypoxia (environmental) , chemistry , biology , gene expression , biochemistry , gene , cdna library , organic chemistry , oxygen

During cardiac muscle development, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcyclin‐binding protein/Siah‐interacting protein (CacyBP/SIP) as one of the candidates being upregulated in the hyperplastic to hypertrophic switch, suggesting an important role of CacyBP/SIP in cardiac development. To show the importance of CacyBP/SIP during myoblast differentiation, we report here that CacyBP/SIP is developmentally regulated in postnatal rat hearts. The overexpression of CacyBP/SIP promotes the differentiation and DNA synthesis of H9C2 cells and primary rat cardiomyocytes, as well as downregulates the expression of β‐catenin. Besides, CacyBP/SIP promotes the formation of myotubes and multinucleation upon differentiation. To investigate the cardioprotective role of CacyBP/SIP in cardiomyocytes, a hypoxia/reoxygenation model was employed. We found that CacyBP/SIP was upregulated during myocardial infarction (MI) and hypoxia/reoxygenation. As a conclusion, CacyBP/SIP may play a role in cardiomyogenic differentiation and possibly protection of cardiomyocytes during hypoxia/reoxygenation injury. J. Cell. Biochem. 98: 555–566, 2006. © 2006 Wiley‐Liss, Inc.

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