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Cyclic AMP‐dependent modification of gonad‐selective TAF II 105 in a human ovarian granulosa cell line
Author(s) -
Wu Yimin,
Lu Yunzhe,
Hu Yanfen,
Li Rong
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20577
Subject(s) - biology , forskolin , activator (genetics) , microbiology and biotechnology , granulosa cell , gene isoform , adenylyl cyclase , medicine , endocrinology , gene , signal transduction , genetics , ovary , stimulation
In response to gonadotropins, the elevated level of intracellular‐cyclic AMP (cAMP) in ovarian granulosa cells triggers an ordered activation of multiple ovarian genes, which in turn promotes various ovarian functions including folliculogenesis and steroidogenesis. Identification and characterization of transcription factors that control ovarian gene expression are pivotal to the understanding of the molecular basis of the tissue‐specific gene regulation programs. The recent discovery of the mouse TATA binding protein (TBP)‐associated factor 105 (TAF II 105) as a gonad‐selective transcriptional co‐activator strongly suggests that general transcription factors such as TFIID may play a key role in regulating tissue‐specific gene expression. Here we show that the human TAF II 105 protein is preferentially expressed in ovarian granulosa cells. We also identified a novel TAF II 105 mRNA isoform that results from alternative exon inclusion and is predicted to encode a dominant negative mutant of TAF II 105. Following stimulation by the adenylyl cyclase activator forskolin, TAF II 105 in granulosa cells undergoes rapid and transient phosphorylation that is dependent upon protein kinase A (PKA). Thus, our work suggests that pre‐mRNA processing and post‐translational modification represent two important regulatory steps for the gonad‐specific functions of human TAF II 105. © 2005 Wiley‐Liss, Inc.