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Low pH and Helicobacter pylori increase nuclear factor kappa B binding in gastric epithelial cells: A common pathway for epithelial cell injury?
Author(s) -
O'Toole Dermot,
AbdelLatif Mohamed M.M.,
Long Aideen,
Windle Henry J.,
Murphy Anne M.,
Bowie Andrew,
O'Neill Luke A.J.,
Weir Donald G.,
Kelleher Dermot
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20539
Subject(s) - helicobacter pylori , electrophoretic mobility shift assay , pathogenesis , microbiology and biotechnology , biology , gastric mucosa , nf κb , inflammation , transcription factor , stomach , immunology , medicine , biochemistry , gene
Helicobacter pylori infection results in peptic ulceration and chronic gastritis through mechanisms which are not fully elucidated. Live H. pylori activate the pro‐inflammatory transcription factor NF‐κB in gastric epithelial cells. Patients may have peptic ulcer disease in the absence of H. pylori infection; therefore other factors contribute to the inflammatory process. Maximal acid output in patients with H. pylori infection and duodenal ulceration is significantly increased indicating a role for acid in the pathogenesis of mucosal ulceration. The effect of low pH on NF‐κB activation in gastric epithelial cells has not been studied. Human gastric epithelial cells (AGS) were exposed to a range of pH changes in the presence or absence of H. pylori . NF‐κB DNA‐binding and cytosolic IκB‐α were measured using electrophoretic mobility shift assay and Western blotting. NF‐κB DNA‐binding in gastric epithelial cells dramatically increased when the pH of the culture medium decreased. Increases in NF‐κB nuclear binding were paralleled by decreasing amounts of cytosolic IκB‐α. These findings were similar but less potent than those observed when cells were exposed to H. pylori . Low pH resulted in enhancement of H. pylori ‐induced NF‐κB nuclear binding. DNA binding of NF‐κB activation secondary to low pH was attenuated by PD98059 but not by SB203580. Similar to H. pylori , low pH potently and independently augments NF‐κB nuclear binding in AGS cells and such activation appears to be mediated through MEK1‐dependant signaling pathways. © 2005 Wiley‐Liss, Inc.