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Andrograpanin, a compound isolated from anti‐inflammatory traditional Chinese medicine Andrographis paniculata , enhances chemokine SDF‐1α‐induced leukocytes chemotaxis
Author(s) -
Ji LiLi,
Wang Zhu,
Dong Fang,
Zhang WenBo,
Wang ZhengTao
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20464
Subject(s) - chemotaxis , cxc chemokine receptors , chemokine , c c chemokine receptor type 6 , andrographis paniculata , xcl2 , chemokine receptor , internalization , ccl5 , cxcr3 , microbiology and biotechnology , ccr2 , stromal cell derived factor 1 , cxcl5 , rutaecarpine , chemistry , jurkat cells , receptor , cxcr4 , inflammation , pharmacology , immunology , biology , medicine , t cell , biochemistry , il 2 receptor , immune system , alternative medicine , pathology
Andrographis paniculata is a traditional Chinese medicine (TCM) that has been effectively used for treatment of infection, inflammation, cold, fever, and diarrhea in China. However, mechanism of its therapeutic function is not well known. In the current study, we showed one of its components, andrograpanin, could enhance chemokine stromal cell‐derived factor‐1α (SDF‐1α) induced chemotaxis in Jurkat and THP‐1 cells. Further study demonstrated that this kind of effect was CXC chemokine receptor‐4 (CXCR4) specific, since andrograpanin could not enhance other chemokines, such as RANTES, monocyte chemotactic protein‐1 (MCP‐1), etc. induced cell chemotaxis. Mechanisms of andrograpanin exerting its effect were not directly in the receptor and G protein coupling level because it had no effect on the binding of SDF‐1 to CXCR4, SDF‐1 induced G protein activation and adenyly cyclase inhibition. However, receptor internalization might be involved, since we found it significantly reduced SDF‐1α‐induced CXCR4 internalization. © 2005 Wiley‐Liss, Inc.