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Increased expression of calcineurin in human colorectal adenocarcinomas
Author(s) -
Lakshmikuttyamma Ashakumary,
Selvakumar Ponniah,
Kanthan Rani,
Kanthan Selliah Chandra,
Sharma Rajendra K.
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20437
Subject(s) - western blot , calcineurin , immunohistochemistry , colorectal cancer , phosphatase , biology , signal transduction , calmodulin , blot , cytoplasm , cancer research , cancer , microbiology and biotechnology , pathology , medicine , enzyme , phosphorylation , immunology , biochemistry , gene , genetics , transplantation
Colorectal cancer (CRC) is the third most common cause of cancer death in the Western world. Calcineurin (CaN), a Ca 2+ /calmodulin (CaM)‐dependent protein phosphatase, is important for Ca 2+ ‐mediated signal transduction. The main objective of this study is to examine the potential role of Ca 2+ /CaM‐dependent protein phosphatase in both normal and in invasive tumor components of human samples. In this study, we carried out 45 cases of CaN activity, 13 cases of CaN protein expression by Western blot analysis, and 6 cases for immunohistochemical analysis in both normal and invasive tumor components of human samples. Immunohistochemical analysis revealed that strong cytoplasmic staining of varying intensity was observed in colon tumors of all patients compared to normal mucosa. In addition, Western blot analysis revealed a prominent overexpressed immunoreactive band with an apparent molecular mass of 60 kDa catalytic alpha subunit (CaN A) as well as CaN Aα and β in colon tumor samples. Elevated CaN protein expression appears to be a possible link between Ca 2+ signaling and oncogenic processes. © 2005 Wiley‐Liss, Inc.