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Serological protein profiling of neuroblastoma by ProteinChip SELDI‐TOF technology
Author(s) -
He QingYu,
Zhu Rui,
Ren Yi,
Tam Paul K.H.,
Chiu JenFu
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20417
Subject(s) - serology , neuroblastoma , biomarker , microbiology and biotechnology , carcinogenesis , biology , antibody , immunology , biochemistry , gene , genetics , cell culture
Serological proteins of neuroblastoma were profiled and analyzed by ProteinChip‐SELDI‐TOF MS technology with five types of protein chips. By comparing with normal control, a number of protein or polypeptide signals were found significantly and consistently different in their intensities (expression levels) in tumor sera. Interestingly, nine polypeptide peaks in these proteomic features can be simultaneously detected with consistent variations by more than one type of protein chips. None of the expression differences of these nine polypeptides was found in similar comparisons between healthy controls and hepatomas. Preliminary protein identification showed hints for that some of these proteomic alterations may be closely related to the tumorigenesis of neuroblastoma. These results demonstrated the potential of serological biomarker identification for neuroblastoma by ProteinChip‐SELDI technology. © 2005 Wiley‐Liss, Inc.