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Spatio‐temporal organization of DNA replication in murine embryonic stem, primary, and immortalized cells
Author(s) -
Panning Margaret M.,
Gilbert David M.
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20395
Subject(s) - biology , embryonic stem cell , microbiology and biotechnology , replication timing , induced pluripotent stem cell , dna replication , stem cell , genetics , gene
The extent to which chromosomal domains are reorganized within the nucleus during differentiation is central to our understanding of how cells become committed to specific developmental lineages. Spatio‐temporal patterns of DNA replication are a reflection of this organization. Here, we demonstrate that the temporal order and relative duration of these replication patterns during S‐phase are similar in murine pluripotent embryonic stem (ES) cells, primary adult myoblasts, and an immortalized fibroblast line. The observed patterns were independent of fixation and denaturation techniques. Importantly, the same patterns were detected when fluorescent nucleotides were introduced into living cells, demonstrating their physiological relevance. These data suggest that heritable gene silencing during commitment to specific cell lineages is not mediated by global changes in the sub‐nuclear organization and replication timing of chromosome domains. © 2004 Wiley‐Liss, Inc.