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Regulation of metastasis‐suppressive gene Nm23 ‐H1 on glycosyl‐transferases involved in the synthesis of sialyl Lewis antigens
Author(s) -
Duan LingLing,
Guo Peng,
Zhang Ying,
Chen HuiLi
Publication year - 2005
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20346
Subject(s) - sialyl lewis x , glycosyltransferase , transfection , sialyltransferase , microbiology and biotechnology , monoclonal antibody , glycan , glycosyl , antigen , chemistry , gene , cell culture , enzyme , antibody , biology , glycoprotein , biochemistry , immunology , adhesion , selectin , genetics , organic chemistry
Abstract By using reverse transcriptase‐polymerase chain reaction (RT‐PCR), the mRNA expressions of three families of glycosyltransferases involved in the synthesis of sialyl Lewis antigens were determined in H7721 human hepatocarcinoma cell line before and after the transfection of metastasis‐suppressive gene nm23 ‐H1. These glycosyltransferases included α1,3fucosyltransferase (α1,3FucT)‐III, ‐IV, ‐VI, ‐VII, and ‐IX, α2,3‐sialyltransferase (ST3Gal)‐I, ‐II, ‐III, and ‐IV as well as O ‐glycan core 2 β1,6 N ‐acetylglucosaminyltransferase (C2GnT)‐I and ‐II. In mock cells transfected with the vector, the expression‐order of α1,3FucTs was IV > VI > III > VII > IX, that of ST3Gals was IV > I > II > III, and that of C2GnT was I > II. Nm23 ‐H1 downregulated the mRNA expressions of all five subtypes of α1,3FucT and ‐I, ‐III, ‐IV subtypes of ST3Gal, but not ST3Gal‐II and C2GnT‐I, II. On the other hand, the expressions of cell surface sialyl Lewis X (SLe x ) and α2,3 sialyl residues were decreased on nm23 ‐H1 transfected cells as detected with monoclonal antibody of SLe x and enzyme‐labeled lectins, respectively. Since SLe x was reported to be a metastasis‐associated glycan structure, the reduced expressions of SLe x and some enzymes related to its synthesis may be one of the mechanisms to explain the metastasis‐suppressive effect of nm23 ‐H1. J. Cell. Biochem. 94: 1248–1257, 2005. © 2005 Wiley‐Liss, Inc.