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Nuclear matrix protein expressions in hepatocytes of normal and cirrhotic rat livers under normal and regenerating conditions
Author(s) -
Yun JingPing,
Liew ChoongTsek,
Chew Eng Ching,
Yin XiaoYu,
Lai Paul Bo San,
Fai Yam Hin,
Li H.K. Richard,
Jin MeiLin,
Ding MingXiao,
Li MingTao,
Lin HanLiang,
Lau Wan Yee
Publication year - 2004
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20034
Subject(s) - liver regeneration , vimentin , cirrhosis , regeneration (biology) , cytokeratin , western blot , microbiology and biotechnology , hepatectomy , chemistry , biology , pathology , immunohistochemistry , medicine , biochemistry , immunology , surgery , resection , gene
We explored the feasibility of studying nuclear matrix protein (NMP) expressions of the hepatocytes in normal and cirrhotic rat livers with liver regeneration after partial hepatectomy. Sixteen Wistar healthy rats were studied with experimental liver regeneration and/or liver cirrhosis. Two‐dimensional (2‐D) gel electrophoresis was used to generate these NMP compositions from these rat liver samples. Several antibodies against cytokeratin, vimentin, actin, B23, HNF4alpha, and heat shock protein 70 were used for identification by Western blot. Totally, 41 strongly stained protein spots were characterized on the 2‐D gels. Thirty‐four protein spots were detected in all of these rat livers, of which, cytokeratin, vimentin, actin, HNF4alpha, and heat shock protein 70 were identified. B23 was detected in the regenerated livers. Three protein spots (s33, s34, and s35) were detectable only in NMP preparation extracted from the regenerating rat livers after hepatectomy. Another three protein spots (s36, s37, and s38) were detectable only in NMP preparation extracted from thioacetamide‐induced cirrhotic rat livers. Under these conditions including experimental liver regeneration and/or liver cirrhosis, Over thirty higher abundance NMPs of hepatocytes were consistently expressed and considered as common and basic NMPs. Some of the NMPs are specific for liver regeneration and may play a critical role in cell proliferation and cell cycle, and some are specific for liver cirrhosis. © 2004 Wiley‐Liss, Inc.