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Heterologous CpG island becomes extensively methylated in the genome of transgenic mice
Author(s) -
Ioudinkova Elena,
Verbovaia Lilia,
Kadulin Sergey,
Goldman Igor,
Razin Sergey V.
Publication year - 2004
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.20023
Subject(s) - heterologous , cpg site , transgene , biology , genome , genetics , dna methylation , computational biology , gene , gene expression
It is demonstrated that a heterologous (chicken) CpG island containing five Sp1 canonical recognition sequences becomes highly methylated in the genome of transgenic mice bearing one or several copies of the transgene. Similar levels of methylation of the chicken CpG island were observed in different tissues of transgenic mice except the brain where the level of methylation of this chicken CpG‐rich fragment was significantly lower than in other tissues. Analysis of susceptibility of the “transgenic” CpG island to Hpa II and Msp I restriction nucleases revealed an unusual methylation pattern interfering with the action of both of these enzymes. A conclusion has been drawn that heterologous CpG island per se does not contain all necessary signals permitting to maintain its own non‐methylated status in the genome of transgenic animals. © 2004 Wiley‐Liss, Inc.