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Changes in the Expression of Membrane Antigens During the Differentiation of Chicken Erythroblasts
Author(s) -
Hayman Michael J.,
Beug Hartmut,
Savin Keith W.
Publication year - 1982
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.1982.240180308
Subject(s) - monoclonal antibody , retrovirus , haematopoiesis , erythroblast , antibody , antigen , hemoglobin , biology , friend virus , mutant , microbiology and biotechnology , cell , transformation (genetics) , chemistry , virus , virology , biochemistry , immunology , gene , stem cell
Chicken erythroblasts can be transformed by the avian retrovirus, avian erythroblastosis virus (AEV). Earlier studies have shown that the mechanism of transformation appears to involve a “block” in differentiation, in that when erythroblasts are transformed by a temperature‐sensitive mutant of ts 34 AEV and incubated at the nonpermissive temperature, the cells start to differentiate and produce hemoglobin. We have decided to use this system to isolate pure populations of chicken erythroblasts and raise monoclonal antibodies against their cell surface proteins. Three monoclonal antibodies were isolated and tested for their ability to bind to various hematopoietic cell types; two were shown to be erythroid‐specific, whereas the other antibody bound to proliferating cells but not to erythrocytes or granulocytes. Of the erythroid‐specific antibodies, one precipitated a 94,000 molecular weight protein, whereas the other precipitated a 11,000 molecular weight protein that was tentatively identified as hemoglobin. The use of this system and approach to identify and evaluate changes that occur during the differentiation is discussed.