Premium
Expression of Adhesion‐Related Membrane Components in Adherent Versus Nonadherent Hamster Melanoma Cells
Author(s) -
Knudsen Karen A.,
Damsky Caroline H.,
Buck Clayton A.
Publication year - 1982
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.1982.240180204
Subject(s) - glycoprotein , adhesion , cell adhesion , membrane glycoproteins , hamster , sodium dodecyl sulfate , chemistry , membrane , neural cell adhesion molecule , cell adhesion molecule , cell culture , gel electrophoresis , cell , microbiology and biotechnology , polyacrylamide gel electrophoresis , biology , biochemistry , enzyme , genetics , organic chemistry
The existence of integral membrane components that are involved in cell–substratum adhesion has been postulated. Using an immunochemical approach developed in this laboratory, we provide further evidence for the role in cell–substratum adhesion of integral membrane glycoproteins within a molecular weight region of 120,000–140,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of material enriched approximately 100‐fold in adhesion‐related components revealed the 120,000–140,000 M r glycoproteins in an adherent hamster melanoma cell line. These glycoproteins are greatly reduced in a non‐adherent variant. Induction of adhesion in these cells by exposure to BudR is accompanied by re‐expression of the surface adhesion antigens.