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Phosphoinositide‐specific phospholipase Cβ1 expression is not linked to nerve growth factor‐induced differentiation, cell survival or cell cycle control in PC12 rat pheocromocytoma cells
Author(s) -
Bortul Roberta,
Aluigi Michela,
Tazzari Pier Luigi,
Tabellini Giovanna,
Baldini Giovanna,
Bareggi Renato,
Narducci Paola,
Martelli Alberto M.
Publication year - 2001
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.1266
Subject(s) - phosphoinositide phospholipase c , phospholipase c , biology , nerve growth factor , microbiology and biotechnology , cytoplasm , phospholipase , cell , nucleus , cell type , gq alpha subunit , cell growth , phospholipase a , cellular differentiation , growth factor , phospholipase a2 , signal transduction , receptor , biochemistry , enzyme , g protein , gene
Recent reports have highlighted that phosphoinositide‐specific phospholipase Cβ1 expression is linked to neuronal differentiation in different experimental models. We sought to determine whether or not this is also true for nerve growth factor (NGF)‐induced neuronal differentiation of rat PC12 cells. However, we did not find differences in the expression of both the forms of phosphoinositide‐specific phospholipase Cβ1 (a and b) during sympathetic differentiation of these cells. Also, PC12 cell clones stably overexpressing phosphoinositide‐specific phospholipase Cβ1 were not more susceptible to the differentiating effect of NGF. Furthermore, since it is well established that phosphoinositide‐specific phospholipase Cβ1 affects cell proliferation, we investigated whether or not PC12 cell clones stably overexpressing phosphoinositide‐specific phospholipase Cβ1 showed differences in survival to serum deprivation and cell cycle, when compared to wild type cells. Nevertheless, we did not find any differences in these parameters between wild type cells and the overexpressing clones. Interestingly, in PC12 cells the overexpressed phosphoinositide‐specific phospholipase Cβ1 did not localize to the nucleus, but by immunofluorescence analysis, was detected in the cytoplasm. Therefore, our findings may represent another important clue to the fact that only when it is located within the nucleus phosphoinositide‐specific phospholipase Cβ1 is able to influence cell proliferation. J. Cell. Biochem. 84: 56–67, 2002. © 2001 Wiley‐Liss, Inc.