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Transcriptional state and chromatin structure of the murine entactin and laminin γ1 genes
Author(s) -
Chen Y.,
Keller J.M.
Publication year - 2001
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.1156
Subject(s) - chromatin , nucleosome , gene , microbiology and biotechnology , biology , laminin , genetics , extracellular matrix
The positions of nucleosomes in the proximal 5′regions of the coordinately regulated murine entactin/nidogen and laminin γ1 genes have been identified in four different transcriptional states—constitutively off, basal, induced, and constitutively induced. In the entactin gene a 450 base pair (bp) region of open chromatin is present between three positioned nucleosomes and the transcriptional start site in the basal, induced, and constitutively induced states. Additionally there is a 200 bp open chromatin region at ∼−2.1 kbp that is only present in the induced and constitutively induced states. In the laminin γ1 gene, a 650 bp region of nucleosome‐free chromatin is present between nucleosomes positioned at ∼ −750 and +120 in all transcriptionally active states. These results suggest that basal co‐expression of these genes requires sites present in these near upstream regions. The induction to high levels appears to involve additional sites and possibly the production of new and/or the modification of existing trans‐acting factors. J. Cell. Biochem. 82: 225–233, 2001. © 2001 Wiley‐Liss, Inc.