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β‐phenylpyruvate and glucose uptake in isolated mouse soleus muscle and cultured C2C12 muscle cells
Author(s) -
BenAbraham Ron,
Gazit Vered,
Vofsi Oded,
BenShlomo Izahar,
Reznick Abraham Z.,
Katz Yeshayahu
Publication year - 2003
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10690
Subject(s) - glut4 , c2c12 , soleus muscle , glucose uptake , glut1 , glucose transporter , myocyte , chemistry , medicine , hypoglycemia , endocrinology , incubation , biochemistry , insulin , skeletal muscle , biology , myogenesis
Previous investigation demonstrated the potential of β‐phenylpyruvate at high concentration to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose‐lowering mechanism, glucose uptake, and quantity of glucose transporters (GLUT1 and GLUT4) in mouse soleus muscle and C2C12 muscle cell lines were investigated following incubation with β‐phenylpyruvate in various concentrations. A marked enhancement of glucose uptake was demonstrated that peaked at 0.5 and 1.0 mM β‐phenylpyruvate in soleus muscle ( P < 0.01) and C2C12 cells ( P < 0.001), respectively. Kinetic analysis in C2C12 cells showed a twofold increase in V max compared with controls ( P < 0.001). In addition, both GLUT1 and GLUT4 levels were increased following exposure to β‐phenylpyruvate. Our findings point to a peripheral hypoglycemic effect of β‐phenylpyruvate. © 2003 Wiley‐Liss, Inc.