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Modification of phospholipids fatty acid composition in reuber H35 hepatoma cells: Effect on HMG‐CoA reductase activity
Author(s) -
GarcíaPelayo M. Carmen,
GarcíaPeregrín Eduardo,
MartínezCayuela Marina
Publication year - 2003
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10650
Subject(s) - polyunsaturated fatty acid , reductase , docosahexaenoic acid , biochemistry , myristic acid , chemistry , fatty acid , enzyme , eicosapentaenoic acid , hmg coa reductase , bovine serum albumin , arachidonic acid , palmitic acid
There is controversy about the effect of saturated and polyunsaturated fats on 3‐hydroxy‐3‐methylglutaryl‐CoA (HMG‐CoA) reductase, the main regulatory enzyme of cholesterogenic pathway. Results from dietary studies are difficult to interpret because diets normally contain a mixture of fatty acids. Therefore, we have used Reuber H35 hepatoma cells whose phospholipids were enriched in different individual fatty acids and have studied their effects on the cellular reductase activity. Lauric, myristic, eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids were supplemented to the culture medium coupled to bovine serum albumin. The four fatty acids were incorporated into phospholipids from cells grown in media containing whole serum or lipoprotein‐poor serum (LPPS). Reductase activity of cells cultivated in a medium with LPPS was three to four times higher than those cultivated in medium with whole serum. Saturated fatty acids increased reductase activity of cells grown in medium with whole serum, whereas n‐3 polyunsaturated fatty acids (PUFA) decreased it. However, both saturated and polyunsaturated fatty acids increased reductase activity when serum lipoproteins were removed. In conclusion, this is one of the first reports demonstrating that saturated and n‐3 PUFA only show differential effects on HMG‐CoA reductase activity in the presence of lipoproteins. © 2003 Wiley‐Liss, Inc.

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