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Critical role of the transcription factor AP‐1 for the constitutive and interferon‐induced expression of IFI 16
Author(s) -
Clarke C.J.P.,
Apostolidis V.,
Hii L.L.P.,
Gough D.J.,
Trapani J.A.,
Johnstone R.W.
Publication year - 2003
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10475
Subject(s) - biology , transcription factor , untranslated region , transcription (linguistics) , gene , gene expression , microbiology and biotechnology , transcriptional regulation , regulation of gene expression , promoter , three prime untranslated region , interferon , genetics , messenger rna , linguistics , philosophy
IFI 16 is a member of the HIN‐200 family of transcriptional regulators that suppress cell growth, modulate the cell cycle and have been linked to cellular differentiation. We hypothesized that the activity of IFI 16 depends on its level of expression and therefore studied the transcriptional activity of the IFI 16 promoter. A discrete sequence within the 5′ untranslated region was required for constitutive activity of the promoter and the functional motif within this region was shown to be a consensus AP‐1 site. Interestingly, this AP‐1 site was also critical for IFN‐induced activation of the promoter and consistent with these observations, treatment of cells with IFNγ resulted in a rapid and robust induction of AP‐1 activity that preceded expression of IFI 16. These experiments define the transcriptional mechanisms of IFI 16 gene regulation and provide evidence suggesting that AP‐1 activation may be an important event in IFN signaling. J. Cell. Biochem. 89: 80–93, 2003. © 2003 Wiley‐Liss, Inc.