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Proteomic analyses of arsenic‐induced cell transformation with SELDI‐TOF ProteinChip® technology
Author(s) -
He QingYu,
Yip TaTung,
Li Muyao,
Chiu JenFu
Publication year - 2002
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10356
Subject(s) - chemistry , microbiology and biotechnology , carcinogenesis , transformation (genetics) , cell , malignant transformation , arsenite , proteomics , neoplastic transformation , arsenic , biology , biochemistry , cancer research , gene , organic chemistry
In this study, we demonstrated that low levels (1.5 μM) of arsenite induces B[a]P‐treated lung cell transformation. We then used a proteomic approach to identify protein expression by ProteinChips, which could potentially be important for transformation induced by this toxic metal. Most of the protein peaks in cell extracts of all samples, including the control, B[a]P‐treated, and B[a]P + As‐treated cells are identical. However, surface‐enhanced laser desorption/ionization time of flight (SELDI‐TOF) analysis with Cu‐ProteinChips and WCX‐ProteinChips revealed several dramatically different protein peaks that appeared in lung cells after being transformed by a treatment of 1.5 μM arsenite for 12 weeks. SAX2 ProteinChip also identified a prominent protein peak that was preferentially expressed in control cells. Interestingly, by using a SAX2 chip, we were able to detect several protein peaks that increased their expression in lung epithelial cells (LEC) treated with only B[a]P. Identification and characterization of these proteins may reveal the molecular basis of As‐induced cell transformation and provide insight into the mechanisms by which arsenic induces carcinogenesis. © 2002 Wiley‐Liss, Inc.