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Osteogenic protein‐1 (OP‐1, BMP‐7) induces osteoblastic cell differentiation of the pluripotent mesenchymal cell line C2C12
Author(s) -
Yeh LeeChuan C.,
Tsai Alicia D.,
Lee John C.
Publication year - 2002
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10315
Subject(s) - bone sialoprotein , osteocalcin , c2c12 , alkaline phosphatase , mesenchymal stem cell , bone morphogenetic protein 2 , microbiology and biotechnology , chemistry , cellular differentiation , cell culture , messenger rna , osteoblast , biology , myocyte , biochemistry , myogenesis , in vitro , genetics , gene , enzyme
The effects of Osteogenic Protein‐1 (OP‐1, BMP‐7) on the differentiation of the pluripotent mesenchymal cell line, C2C12, were examined. OP‐1 at 50 ng/ml partially inhibited myotube formation in C2C12 cells, while OP‐1 at 200 ng/ml completely inhibited myotube formation and induced the formation of cells displaying osteoblastic morphology. High concentrations of OP‐1 elevated the alkaline phosphatase (AP) activity dramatically, both as a function of time and OP‐1 concentration. Osteocalcin (OC) mRNA expression was detected as early as 8 days in OP‐1‐treated cultures and subsequently increased considerably. Expression of bone sialoprotein (BSP) mRNA was low in control cultures and stimulated by OP‐1. Collagen type I mRNA expression was enhanced by OP‐1 during the early days in culture, but gradually decreased thereafter. MyoD mRNA expression, high in control cultures, was suppressed by OP‐1 in a dose‐ and time‐dependent manner. OP‐1 enhanced ActR‐I mRNA expression and significantly elevated the mRNA expressions of BMP‐1, BMP‐4, BMP‐5, GDF‐6, and GDF‐8. The present results indicate that OP‐1 is a potent inducer of C2C12 differentiation into osteoblastic cells. J. Cell. Biochem. 87: 292–304, 2002. © 2002 Wiley‐Liss, Inc.