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DNase I sensitive site in the core region of the human β‐globin origin of replication
Author(s) -
Djeliova Vera,
Russev George,
Anachkova Boyka
Publication year - 2002
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10298
Subject(s) - chromatin , microbiology and biotechnology , biology , nuclear matrix , deoxyribonuclease i , dnase i hypersensitive site , hypersensitive site , dna replication , globin , dna , gene , genetics , base sequence
HeLa cells were synchronized at late G1, early S, and late S phase of the cell cycle by nocodazole treatment. The cells were permeabilized with Triton X‐100, digested with DNAse I, and extracted with 0.2 M ammonium sulfate to remove the digested chromatin. DNA was isolated from the residual chromatin attached to the nuclear matrix, digested with Hind III, and subjected to hybridization with [ 32 P] labeled probe located upstream of the core region of the human β‐globin replication origin. The hybridization pattern revealed the existence of a DNase I sensitive site in the core region of the β‐globin replicator. The results suggest that association with the nuclear matrix induce alteration in the chromatin structure of the origin of replication that represents a more open chromatin configuration. J. Cell. Biochem. 87: 279–283, 2002. © 2002 Wiley‐Liss, Inc.