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Gene expression for a novel protein RGPR‐p117 in various species: The stimulation by intracellular signaling factors
Author(s) -
Misawa Hiroyuki,
Yamaguchi Masayoshi
Publication year - 2002
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10289
Subject(s) - northern blot , messenger rna , xenopus , gene , gene expression , microbiology and biotechnology , biology , intracellular , western blot , biochemistry
The presence and expression for the gene encoding a novel regucalcin gene promoter region‐related protein (RGPR‐p117) in various species was investigated by using Southern “zoo blot” and Northern hybridization analyses. A “zoo blot” analysis demonstrated that RGPR‐p117 gene was widely conserved in various species including human, rat, mouse, dog, cow, pig, rabbit, chicken, fish, C. elegans and yeast. The gene was not found in Xenopus . Northern blot analysis showed that RGPR‐p117 mRNA was expressed in the liver of human, rat, mouse, and rabbit as a single mRNA of ∼4.5 kb, respectively. However, homologous mRNA was not found in the liver of Xenopus . The expression of RGPR‐p117 mRNA in liver was clearly enhanced 5 h after a single intraperitoneal administration of CaCl 2 (5 mg Ca 2+ /100 g body weight) to rats. The RGPR‐p117 mRNA is also expressed in the cloned H4‐II‐E rat hepatoma cells, although this expression was weak as compared with that of liver tissues. Moreover, the RGPR‐p117 mRNA expression in H4‐II‐E cells was stimulated in the presence of dibutyryl cAMP, PMA, insulin, 17β‐estradiol, or serum in culture medium. The present study demonstrates that the RGPR‐p117 gene is conserved in various species, and that its expression is stimulated by intracellular signaling factors. J. Cell. Biochem. 87: 188–193, 2002. © 2002 Wiley‐Liss, Inc.