Premium
Identification of cultured progenitor cells from human marrow stroma
Author(s) -
Shur I.,
Marom R.,
Lokiec F.,
Socher R.,
Benayahu D.
Publication year - 2002
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10267
Subject(s) - stromal cell , flow cytometry , progenitor cell , microbiology and biotechnology , biology , haematopoiesis , bone marrow , cell cycle , ex vivo , cell , stem cell , stroma , immunology , in vivo , cancer research , immunohistochemistry , biochemistry
The marrow stromal cells (MSC) are essential for regulation of bone remodeling and hematopoiesis. It is of prime importance to isolate MSC and to expand the proliferating cells ex vivo. In this study, we analyzed cultured MSC for various cellular parameters, including cell morphology, cell cycle, and expression of cell surface antigens by flow cytometry. MSC were divided based on cell size to small (S‐cells) and large (L‐cells) and were visualized by light and electron microscope. The S‐cells were proliferating cells correlated with G 0 /G 1 phase of cell cycle, and expressed cFOS. The expression of surface markers CD‐34, ‐44, ‐51, ‐61, ‐62E, ‐62P, ‐62L was quantified using flow cytometry. CD‐44 was ubiquitously expressed by S and L cells, CD‐51 and ‐61 were expressed by 30%–38% of S‐cells. CD‐34 and ‐62 expressed 20% positive of the analyzed cells that were of the proliferating progenitors (S‐cells). This study enables the identification of subpopulations from MSC with special attention paid to the proliferating cells from ex vivo cultures of marrow stroma. J. Cell. Biochem. 87: 51–57, 2002. © 2002 Wiley‐Liss, Inc.