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Identifying and modifying protein–DNA and protein–protein interactions using a bacterial two‐hybrid selection system
Author(s) -
Joung J. Keith
Publication year - 2001
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.10065
Subject(s) - dna , bacteria , yeast , biology , selection (genetic algorithm) , bacterial protein , computational biology , protein–protein interaction , microbiology and biotechnology , biochemistry , genetics , computer science , artificial intelligence
A bacterial two‐hybrid system based on transcriptional activation in E. coli has recently been described. A variety of different protein–DNA and protein–protein interactions from bacteria, yeast, and humans have been studied using this bacterial‐based system. The method, because it is based in bacteria, offers significant advantages relative to its yeast counterpart including the ability to analyze complex libraries > 10 8 in size, ease of use, and speed. The ability to easily and rapidly process very large libraries make this system a powerful tool for identifying, modifying, or optimizing protein–DNA and protein–protein interactions. J. Cell. Biochem. Suppl. 37: 53–57, 2001. © 2002 Wiley‐Liss, Inc.