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Preparation and storage of “leukocyte‐depleted single donor” platelet concentrate: A step forward in effective haemotherapy
Author(s) -
Elias Mona K.,
de Wolf Joost Th. M.,
Blom Nel,
Rijskamp Luit,
Halie Ruud M.,
Sibinga Cees Th. Smit
Publication year - 1991
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.2920060304
Subject(s) - lactic dehydrogenase , platelet , medicine , elutriation , lactate dehydrogenase , platelet concentrate , lactic acid , surgery , immunology , andrology , biochemistry , chemistry , enzyme , organic chemistry , biology , bacteria , genetics
“Leukocyte‐depleted” platelet concentrates (LD‐SDPC) were prepared by cotton‐wool and by polyester filtration of “leukocyte‐poor” PC collected by the elutriation technique. The storage characteristics of LD‐SDPC were comparable to those of the filtered pools of multiple donor platelet concentrates (LD‐MDPC). However, LD‐SDPC were less activated and less damaged than LD‐MDPC over a 7‐day storage period, as evidenced by β‐thromboglobin percent release and lactic dehydrogenase percent leakage in both products. LD‐SDPC were prophylactically transfused to 12 thrombocytopenic patients; the mean bleeding time was shortened from 12 min 20 sec to 4 min 39 sec. Corrected count increment (CCI) was 22.8 at 1 hr and 14.1 at 24 hr compared to 15.5 and 10.0, respectively, with standard PCs.