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Concentration of activated t lymphocytes in extracorporeal blood circulation for plasma separation
Author(s) -
Ijichi Shinji,
Mishima Masaki,
Matsuda Takemasa,
Izumihara Tomomaro,
Kojima Kazuhisa,
Niimura Tatsuru,
Maruyama Ikuro,
Osame Mitsuhiro
Publication year - 1991
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.2920060206
Subject(s) - medicine , immunofluorescence , extracorporeal circulation , extracorporeal , flow cytometry , cd8 , rheumatoid arthritis , immunology , antigen , antibody
To investigate whether the proportion of activated T lymphocytes changes in an extracorporeal blood flow, we compared paired samples collected at the inlet and outlet lines of an artificial circuit for plasma separation, using a dual‐immunofluorescence flow cytometric technique. In our series of materials from patients with rheumatoid arthritis or systemic lupus erythematosus, the percentages of HLA‐DR‐positive cells in both CD4 + ( P < 0.01, n = 6) and CD8 + ( P < 0.05. n = 10) subsets were significantly increased in the outlet lines compared with the inlet lines, suggesting that activated T lymphocytes pass through an extracorporeal blood circuit more easily than resting T lymphocytes.