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Concurrent comparison of the cobe spectra and fenwal CS3000 for the collection of peripheral blood mononuclear cells for autologous peripheral stem cell transplantation
Author(s) -
Padley Doug,
Strauss Ronald G.,
Wieland Martha,
Randels M. Joleen
Publication year - 1991
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.2920060203
Subject(s) - leukapheresis , peripheral blood mononuclear cell , medicine , transplantation , bone marrow , haematopoiesis , hematopoietic stem cell transplantation , platelet , blood cell , stem cell , nuclear medicine , andrology , immunology , cd34 , biology , in vitro , biochemistry , genetics
Hematopoietic stem cells, collected by leukapheresis from peripheral blood, can be used as an alternative to autologous bone marrow transplantation following high‐dose chemotherapy as treatment of several malignancies. We compared the ability of the Cobe Spectra and the Fenwal CS3000 to collect peripheral blood mononuclear cells (MNC) for autologous peripheral blood stem cell transplantation. Ten patients experienced repeated leukapheresis (10 L blood processed per procedure) using both instruments. Procedures were alternated between the two until a total of 7 × 10 8 MNC/kg was collected. Data from 61 Spectra and 50 CS3000 collections were analyzed. The yield (mean per procedure) of nucleated cells (NC) and MNC was higher ( P < .005) with the Spectra (0.77 × 10 10 NC and 0.54 × 10 10 MNC) than with the CS3000 (0.59 × 10 10 NC and 0.40 × 10 10 MNC). However, colony forming units (CFU‐GM) were not different ( P > .05) for Spectra (0.92 × 10 4 ) and Fenwal (0.65 × 10 4 ) collections. Platelet contamination was lower ( P < .001) with the Spectra (2.2 × 10 11 ) than the CS3000 (5.0 × 10 11 ). This correlated with a higher patient blood platelet count immediately following Spectra collections (117 × 10 9 /L) versus the CS3000 (86 × 10 9 /L). Using the methods described, the Spectra product contained greater yields of NC and MNC with less platelet contamination than did the CS3000.