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Peripheral blood lymphocyte numbers, lymphocyte proliferative responses in vitro, and serum immunoglobulins in regular hemapheresis donors
Author(s) -
Braine Hayden G.,
Elfenbein Gerald J.,
Mellits E. David
Publication year - 1985
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.2920020302
Subject(s) - immunology , medicine , lymphocyte , plateletpheresis , pokeweed mitogen , population , antibody , antigen , leukapheresis , peripheral blood mononuclear cell , in vitro , apheresis , biology , platelet , stem cell , biochemistry , genetics , environmental health , cd34
Selected tests of lymphoid function were used to screen a population of volunteer hemapheresis donors. Testing included: 1) absolute lymphocyte numbers, and percentage of T‐cell, B‐cell, and mononuclear phagocytes, 2) serum immunoglobulins, and, 3) in vitro proliferative responses to lectin mitogens (phytohemagglutinin, concana‐valin A, and pokeweed mitogen), soluble antigens (staphylococcal filtrate, Candida, and streptococcal varidasc), and cell‐bound alloantigens (mixed lymphocyte culture). A control population of first‐time plateletpheresis donors was examined similarly. Regular donors manifested a small but statistically significant decrease in absolute lymphocyte counts (p < 0.02), and IgM (p < 0.02) compared to controls. Leukapheresis donors also manifested significant decreases in percentage of T cells (p < 0.02). These findings are qualitatively similar to changes reported following intensive lymphocytapheresis and indicate the need for conservative policies regarding donation frequency in hemapheresis programs.