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Granulocyte collection by continuous‐flow centrifugation using arteriovenous shunts
Author(s) -
Williams Barbara M.,
Clift R. A.,
Buckner C. Dean,
Hickman Robert O.,
Sanders Jean E.,
Stewart Patricia S.,
Bensinger William I.,
Banaji Meera,
Thomas E. Donnall
Publication year - 1984
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.2920020211
Subject(s) - medicine , granulocyte , leukapheresis , silastic , surgery , hydroxyethyl starch , transplantation , anesthesia , cd34 , biology , stem cell , genetics
Two thousand nine hundred twenty‐seven granulocyte collections were made using continuous‐flow centrifugation with the Aminco Celltrifuge I. the Fenwal Celltrifuge II. and the IBM 2997. There were 231 recipients and 246 donors. In 32 patients attempts to provide daily granulocyte transfusions from a single donor were unsuccessful owing to clotting of the silastic‐Teflon arteriovenous shunt. Repeated granulocyte donations produced severe anemia requiring red cell transfusions in all the females and most of the males who had previously donated marrow for transplantation. Granulocyte collections were similar when collected with the Celltrihge I or the IBM 2997. but collections with the Celltrifuge Il were smaller. Infusion of hydroxycthyl starch directly into whole blood as it entered the centrifuge increased granulocyte collections. Granulocyte collections decreased with increasing number of daily collections.