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An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft‐vs‐host‐disease in a low body weight pediatric patient
Author(s) -
Matic Toni,
Bojanic Ines,
Mazic Sanja,
Golubic Cepulic Branka,
Bilic Ernest
Publication year - 2019
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.21700
Subject(s) - buffy coat , medicine , extracorporeal photopheresis , extracorporeal , venipuncture , surgery , photopheresis , graft versus host disease , disease , transplantation
A mini extracorporeal photopheresis (mini‐ECP) “off line” technique has been developed for use in the treatment of small children and patients with apheresis contraindications. Until now various methods have been used for buffy coat separation from whole blood. In this report we describe a protocol for mini buffy coat preparation using the automated Sepax laboratory separator for “off line” ECP treatment in a low body weight child with graft‐vs‐host‐disease. According to our results this alternative method has been proven feasible and tolerable.