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Performance prediction algorithm for autologous PBSC collection in adults and pediatric patients using large volume leukapheresis
Author(s) -
Bojanic Ines,
Besson Nelly,
Vidovic Ivana,
Cepulic Branka Golubic
Publication year - 2019
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.21693
Subject(s) - medicine , leukapheresis , apheresis , cd34 , interquartile range , urology , nuclear medicine , peripheral blood mononuclear cell , surgery , stem cell , platelet , genetics , biology , biochemistry , chemistry , in vitro
Background and Objectives The number of CD34+ cells collected in apheresis procedures depends mainly on the collection efficiency of the device and the blood volume processed. Large volume leukapheresis (LVL) can improve CD34+ cell yield and has previously been investigated using the COBE Spectra device (Terumo BCT, USA). Materials and Methods This was a retrospective analysis of LVL performance in patients undergoing continuous mononuclear cell collection (CMNC) using the new Spectra Optia apheresis system (Terumo BCT, USA) at the University Hospital Center, Zagreb, from March 2016 to September 2016. CD34+ cell yield predictability, determined using a customized algorithm, was also assessed. Results In total, 67 procedures performed in 46 adults and 14 performed in 11 children were included in the analysis. In adults, 30 (65.2%) patients successfully reached their target preapheresis CD34+ cell count on day 1, with a median (interquartile range [IQR]) CD34+ collected cell dose of 4.8 × 10 6 /kg (2.3‐10.6 × 10 6 /kg). In the pediatric group, 81.8% successfully collected the target CD34+ cell dose on the first day, with a median (IQR) CD34+ collected cell dose of 11.1 × 10 6 /kg (3.2‐16.3 × 10 6 /kg). The customized algorithm showed a strong and significant linear correlation with actual CD34+ cell dose ( P < 0.0001). Conclusion The results of this study support the use of LVL and the customized prediction algorithm in apheresis procedures. The ability to tailor the procedure to meet the needs of the individual patient may help to minimize the blood volume processed, shorten the duration, reduce the volume of infused anticoagulants, and improve patient comfort.