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G‐CSF mobilized peripheral blood stem cell collection for allogeneic transplantation in healthy donors: Analysis of factors affecting yield
Author(s) -
Machaczka Maciej,
Hägglund Hans,
Staver Emma,
Joks Monika,
Hassan Moustapha,
Wahlin Björn Engelbrekt,
Axdorph Nygell Ulla
Publication year - 2017
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.21524
Subject(s) - medicine , cd34 , transplantation , andrology , yield (engineering) , peripheral blood , apheresis , immunology , multivariate analysis , stem cell , urology , biology , platelet , genetics , materials science , metallurgy
Mobilized PBSC are the main source for allogeneic HSCT. We aimed to evaluate factors that affect CD34+ cell yield including the donor's age, gender, BSA, processed blood volume and the method of G‐CSF dose calculation. Data from 170 healthy donors were analyzed. The concentration of CD34+ cells in the peripheral blood (PB) and the processed volume of blood were significantly correlated to CD34+ cells yield ( P < .00005 and P < .001, respectively). The G‐CSF dose per m 2 was significantly correlated to the concentration of CD34+ cells in the PB ( P = .0003) and in the product ( P = .01). Smaller BSA and less processed volume were found among female donors, who were given lesser G‐CSF dose per m 2 , and showed lower yield compared to men. However, multivariate analysis of the yield showed that only the concentration of CD34+ cells in the PB and the processed volume remained independent significant.