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Effective collection of peripheral blood stem cells in children weighing 20 kilogram or less in a single large‐volume apheresis procedure
Author(s) -
SalazarRiojas Rosario,
GarcíaLozano José Alberto,
ValdésGalván Mayra,
MartínezGonzález Odra,
CantúRodríguez Olga Graciela,
GonzálezLlano Oscar,
GómezDe León Andrés,
JaimePérez José Carlos,
GómezAlmaguer David,
GutiérrezAguirre César Homero
Publication year - 2015
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.21375
Subject(s) - medicine , apheresis , surgery , transplantation , cd34 , blood volume , stem cell , platelet , biology , genetics
: Peripheral blood stem cell (PBSC) transplantation has become a routine procedure in pediatric oncology. A special group of PBSC donors are children weighing 20 kg or less. Limited vascular access and low blood volume puts them at a higher risk. Central line placement and a priming apheresis machine are recommended to avoid these complications. Patients and Methods : PBSC collections performed from July 2006 to May 2013 in children weighing less than 20 kg were included. All donors had a central venous catheter (CVC). An apheresis machine was primed with packet red blood cells. Results : Twenty‐seven PBSC collections were performed in 22 children weighing 20 kg or less, 14 for allogeneic and 8 for autologous transplantation, in order to collect at least 2 × 10 6 CD34+ cells/kg. In the allogeneic group, median age and weight were 3 years (0.8–7) and 15.5 kg (8–20). In the autologous group, median age and weight were 3 years (2–7) and 15.35 kg (12.5–19.5). A single large‐volume apheresis was sufficient to obtain the CD34+ cells needed in 78.5% and 75% of the allogeneic and autologous groups, respectively, with a median 11.84 × 10 6 and 5.79 × 10 6 CD34+ cells collected per kilogram of weight of the recipient. No serious complications related to the apheresis procedure or CVC placement occurred. Conclusion : PBSC collection in a single large‐volume apheresis for allogeneic and autologous transplants in children weighing 20 kg or less is a safe and effective procedure when based on standardized protocols. J. Clin. Apheresis 30:281–287, 2015. © 2014 Wiley Periodicals, Inc.