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CD34 + collection efficiency as a function of blood volumes processed in pediatric autologous peripheral blood stem cell collection
Author(s) -
Dubrovsky Leonid,
Wong Edward C.C.,
PerezAlbuerne Evelio,
Loechelt Brett,
Kamani Naynesh,
Sande Jane,
Mintz Kathy,
Paul Wendy,
Luban Naomi L.C.,
Rood Brian R.,
Fry Terry
Publication year - 2011
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.20281
Subject(s) - medicine , apheresis , neuroblastoma , granulocyte colony stimulating factor , cd34 , haematopoiesis , surgery , urology , stem cell , chemotherapy , platelet , biology , genetics , cell culture
Purpose: To characterize the relationship between CD34 + collection efficiency and blood volumes processed in pediatric patients undergoing autologous peripheral blood stem cell (PBSC) collection. Methods: Retrospective 8‐year (2001–2009) study of pediatric patients ( n = 79) with neuroblastoma and central nervous system (CNS) tumors undergoing first day of autologous PBSC harvest using MNC program on the COBE Spectra (Caridian BCT, Lakewood, CO) was performed. Patients undergoing 0 to 2.9 BV (standard volume), 3 to 6 BV (large volume), and greater than 6 BV (ultra large volume) harvests were evaluated for CD34 + collection efficiency, diagnosis (neuroblastoma vs. nonneuroblastoma), disease type (primary vs. relapse), mobilization regimen, granulocyte colony stimulating factor (GCSF) dose, and apheresis complications. Results: CD34 + collection efficiencies (CE) for neuroblastoma patients were 67%, 50%, and 53% for standard ( n = 14), large ( n = 9), and ultra large ( n = 5) volume harvests, respectively. Similarly, patients with nonneuroblastoma diagnoses had CD34 + CE of 63%, 55%, and 65% for low ( n = 19), large ( n = 27), and ultra large ( n = 5) volume harvests, respectively. Weight, granulocyte colony stimulating factor (G‐CSF) stimulation, type of mobilization, and apheresis complications (normalized by run time) were similar between the standard, large, and ultra large volume groups in patients with either neuroblastoma or nonneuroblastoma diagnoses. Conclusions: CD34 + collection efficiency in pediatric autologous PBSC collection on the first day of harvest does not decrease with higher numbers of blood volumes processed in patients with either neuroblastoma or nonneuroblastoma primary disease. These results indirectly indicate bone marrow CD34 + cell mobilization occurs with longer apheresis procedures in pediatric patients. J. Clin. Apheresis, 2011. © 2011 Wiley‐Liss, Inc.