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Postthaw clotting of peripheral blood stem cell products due to insufficient anticoagulant
Author(s) -
ReichSlotky Ronit,
Patel Nita,
Dael Shatrina,
SemideiPomales Mildred,
Stephens Hannah,
Reich Michael,
Schwartz Joseph
Publication year - 2009
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.20220
Subject(s) - medicine , apheresis , blood product , anticoagulant , clotting factor , blood clotting , surgery , platelet , immunology
Abstract The amount of acid citrate dextrose formula A (ACD‐A), which is a commonly used anticoagulant in leukopheresis, has to ensure both the safety of the donor and guarantee the integrity of the peripheral blood stem cell (PBSC) product until its transplant. Two recent consecutive cases of postthaw PBSC product clotting initiated a look‐back investigation of the ACD‐A percentage in leukopheresis products collected in our facility. The data indicated a significant difference between the average amount of ACD‐A in prefreezing products collected during 2006 (11.4%) and in products collected during 2007 and 2008 (8.8% and 8.7%, respectively). These findings and the fact that the two clotted products had less than 7% ACD‐A indicated that insufficient amount of anticoagulant might contribute to their clotting. This investigation prompted us to modify our collection and thawing procedures to prevent similar events in the future. J. Clin. Apheresis 2009. © 2009 Wiley‐Liss, Inc.