Premium
Predictive parameters for granulocyte colony‐stimulating factor‐induced peripheral blood stem cell mobilization
Author(s) -
Okano Akira,
Ashihara Eishi,
Shimazaki Chihiro,
Uchiyama Hitoji,
Inaba Tohru,
Taniguchi Kyoko,
Maekawa Taira,
Taniwaki Masafumi
Publication year - 2008
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.20179
Subject(s) - leukapheresis , granulocyte colony stimulating factor , medicine , cd34 , white blood cell , immunology , stem cell , granulocyte , transplantation , stem cell factor , andrology , progenitor cell , haematopoiesis , chemotherapy , biology , microbiology and biotechnology
To improve the selection of donors for allogeneic stem cell transplantation, it is important to identify reliable parameters that predict CD34+‐cell yields after granulocyte‐colony stimulating factor (G‐CSF)‐induced peripheral blood stem cell (PBSC) mobilization. We retrospectively investigated the peripheral blood (PB) kinetics of white blood cells (WBCs), CD34+ cells, matrix metalloproteinases (MMP)‐9 and ‐2, and tissue inhibitors of metalloproteinases (TIMP)‐1 and ‐2 in 15 healthy donors during their treatment with G‐CSF. All donors received 10 μg/kg of recombinant human G‐CSF once a day subcutaneously. Leukapheresis was initiated after 4 days of G‐CSF treatment, and G‐CSF treatment continued until the last day of leukapheresis. WBC and CD34+ cell numbers in the PB rose after 2 and 3 or 4 days of G‐CSF treatment, respectively. The PB CD34+ cell numbers on day 4 correlated weakly with the increase in WBC counts from day 1 to day 2 ( R 2 = 0.254, P = 0.056). There were also positive correlations between the CD34+ cell numbers in the PBSC products on day 4 and the CD34+ cells in the PB on days 1 and 4 ( R 2 = 0.768, P < 0.0001 and R 2 = 0.816, P < 0.0005, respectively). The MMP‐9 plasma levels on days 1 and 4 also correlated positively with the day 4 circulating CD34+ cell numbers ( R 2 = 0.393, P < 0.05 and R 2 = 0.406, P = 0.01, respectively). In conclusion, the CD34+ cell numbers in the PB steady state may be a useful parameter selecting allogeneic PBSC donors. J. Clin. Apheresis, 2008. © 2008 Wiley‐Liss, Inc.