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Clinical impact of a new automated system employed for peripheral blood stem cell collection
Author(s) -
Del Fante Claudia,
Perotti Cesare,
Viarengo Gianluca,
Bellotti Laura,
Parisi Cristina,
Marchesi Andrea,
Tinelli Carmine,
Salvaneschi Laura
Publication year - 2006
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.20102
Subject(s) - medicine , peripheral blood , stem cell , intensive care medicine , immunology , pathology , biology , genetics
At the moment, PBSC collections can be performed using semi‐automated or automated cell separator devices. The automated methods offer the advantages of a decreased working load for dedicated personnel and high standardization of the collection procedure. Herein we report our single institutional experience in 80 PBSC collections employing the new automated COM.TEC Fresenius autoMNC program that provides the ability to predict the total number of CD34+ cells collected, based on the pre‐leukapheresis CD34+ cell count in peripheral blood. Fourty‐eight patients and 21 healthy donors were mobilized with chemotherapy + G‐CSF or G‐CSF alone, respectively. Eighty leukapheresis collections were performed starting with a CD34+ cell count in peripheral blood at least of 20/μL. Collection parameters and related side effects were evaluated. The mean CD34+ cell collection efficiency in patients and donors was 81.8% (sd 27.6) and 95.1% (sd 15.6), respectively. The mean difference between real and predicted CD34+ cells was +30.2% (sd 92.9) for patients and +4.6% (sd 30.3) for donors. The mean leukapheresis bag volume was 240.7 ml (sd 67.3) and 310.3 ml (sd 86.8) with a mean HCT of 10.9% (sd 34.4) and 9.2% (sd 3.9) for patients and donors, respectively. The automated PBSC collection with the new program COM.TEC Fresenius autoMNC demonstrated a very high CD34+ cell collection efficiency. Moreover, the ability to predict the CD34+ cell yield permits improved management of the leukapheresis collection, with the only disadvantage of larger leukapheresis volume and higher hematocrit. J. Clin. Apheresis © 2006 Wiley‐Liss, Inc.