Premium
Assessment of complement activation during membrane‐based plasmapheresis procedures
Author(s) -
Burnouf Thierry,
Eber Michel,
Kientz Daniel,
Cazenave JeanPierre,
Burkhardt Thomas
Publication year - 2004
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.20019
Subject(s) - anaphylatoxin , plasmapheresis , centrifugation , complement system , chromatography , membrane filter , filtration (mathematics) , medicine , membrane , fresh frozen plasma , apheresis , andrology , chemistry , immunology , biochemistry , antibody , platelet , statistics , mathematics
Previous studies have suggested that plasmapheresis procedures using a separation membrane may activate the complement system and release anaphylatoxins. This study determines the content in C3a/C3a des Arg and C5a/C5a des Arg in plasma donations obtained by the new Haemonetics® Filter Core (FC) procedure and compares it to Baxter Autopheresis C ® (Auto‐C). FC performs sequential blood centrifugation and plasma filtration on a microporous polyethersulfone membrane, while Auto‐C removes blood cells by simultaneous gravitation and filtration on a rotating nylon membrane. One group of 34 donors donated on FC and two groups of 30 and 10 donors on Auto‐C. Plasma aliquots were taken from the plasma units within 30 min of the end of the collection procedures, frozen at <−30°C and assessed for C3a and C5a at various time points of storage. Mean C3a/C3a des Arg in FC plasma (N = 34) was 1,151 (range: 526–2,991), 1,092 (range: 349–3498), and 507 (range: 307–815) ng/ml at time of collection and after 6 and 12 months of storage, respectively. Respective C5a/C5a des Arg was 26.6 (range 4.9–74), 18.9 (9.5–42.6), and 30.9 (range: 10.7–62.3) ng/ml. Mean C3a/C3a des Arg was higher in Auto‐C ( P < 0.001): 4,724 ng/ml (N = 10; range: 2,400–7 ,360) and >4,149 ng/ml (N = 30; 2,408–>6,430) after 3 and 18 months storage, respectively. Mean C5a/C5a des Arg was 32.1 ng/ml (N = 30; range: 10.6–57.2) after 18 months of storage. Complement activation in FC plasmas appears limited compared to Auto‐C, suggesting better biocompatibility of this collection device and/or a favourable impact of the sequential cell centrifugation/filtration technology used. Further studies are needed to explain differences in complement activation between apheresis procedures and to assess clinical impacts, if any. J. Clin. Apheresis, 19:142–147, 2004. © 2004 Wiley‐Liss, Inc.